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Tests and Procedures In vitro fertilization (IVF)


In vitro fertilization (IVF) is a complex series of procedures used to treat fertility or genetic problems which assist with the conception of a child. During IVF, mature eggs are collected (retrieved) from your ovaries and fertilized by your husband sperm in IVF lab. Then the fertilized egg (embryo) or embryos are implanted in your uterus. One cycle of IVF takes about two weeks.

IVF is the most effective form of assisted reproductive technology.

Your chances of having a healthy baby using IVF depend on many factors, such as your age and the cause of infertility.

If more than one embryo is implanted in your uterus, IVF can result in a pregnancy with more than one fetus (multiple pregnancy).

Your doctor can help you understand how IVF works, the potential risks and whether this method of treating infertility is right for you.

Why it's done

In vitro fertilization (IVF) is a treatment for infertility or genetic problems.

Occasionally, IVF is offered as a primary treatment for infertility in women over age 40. IVF can also be done if you have certain health conditions. For example, IVF may be an option if you or your partner has:

•        Fallopian tube damage or blockage. Fallopian tube damage or blockage makes it difficult for an egg to be fertilized or for an embryo to travel to the uterus.

•        Ovulation disorders. If ovulation is infrequent or absent, fewer eggs are available for fertilization.

•        Premature ovarian failure. Premature ovarian failure is the loss of normal ovarian function before age 40. If your ovaries fail, they don't produce normal amounts of the hormone estrogen or have eggs to release regularly.

•        Endometriosis. Endometriosis occurs when the uterine tissue implants and grows outside of the uterus — often affecting the function of the ovaries, uterus and fallopian tubes.

•        Uterine fibroids. Fibroids are benign tumors in the wall of the uterus and are common in women in their 30s and 40s. Fibroids can interfere with implantation of the fertilized egg.

•        Previous tubal sterilization or removal. If you've had tubal ligation — a type of sterilization in which your fallopian tubes are cut or blocked to permanently prevent pregnancy — and want to conceive, IVF may be an alternative to tubal ligation reversal.

•        Impaired sperm production or function. Below-average sperm concentration, weak movement of sperm (poor mobility), or abnormalities in sperm size and shape can make it difficult for sperm to fertilize an egg. If semen abnormalities are found, your partner might need to see a specialist to determine if there are correctable problems or underlying health concerns.

•        Unexplained infertility. Unexplained infertility means no cause of infertility has been found despite evaluation for common causes.

•        A genetic disorder. If you or your partner is at risk of passing on a genetic disorder to your child, you may be candidates for preimplantation genetic diagnosis — a procedure that involves IVF. After the eggs are harvested and fertilized, they're screened for certain genetic problems, although not all genetic problems can be found. Embryos that don't contain identified problems can be transferred to the uterus.


•        Fertility preservation for cancer or other health conditions.If you're about to start cancer treatment — such as radiation or chemotherapy — that could harm your fertility, IVF for fertility preservation may be an option. Women can have eggs harvested from their ovaries and frozen in an unfertilized state for later use. Or the eggs can be fertilized and frozen as embryos for future use.



Specific steps of an in vitro fertilization (IVF) cycle carry risks, including:

•        Multiple births. IVF increases the risk of multiple births if more than one embryo is implanted in your uterus. A pregnancy with multiple fetuses carries a higher risk of early labor and low birth weight than pregnancy with a single fetus does.

•        Premature delivery and low birth weight. Research suggests that use of IVF slightly increases the risk that a baby will be born early or with a low birth weight.

•        Ovarian hyperstimulation syndrome. Use of injectable fertility drugs, such as human chorionic gonadotropin (HCG), to induce ovulation can cause ovarian hyperstimulation syndrome, in which your ovaries become swollen and painful. Signs and symptoms typically last a week and include mild abdominal pain, bloating, nausea, vomiting and diarrhea. If you become pregnant, however, your symptoms might last several weeks. Rarely, it's possible to develop a more severe form of ovarian hyperstimulation syndrome that can also cause rapid weight gain and shortness of breath.

•        Miscarriage. The rate of miscarriage for women who conceive using IVF with fresh embryos is similar to that of women who conceive naturally — about 15 to 20 percent — but the rate increases with maternal age. Use of frozen embryos during IVF, however, may slightly increase the risk of miscarriage.

•        Birth defects. The age of the mother is the primary risk factor in the development of birth defects, no matter how the child is conceived. Experts believe that the use of IVF does not increase the risk of having a baby with birth defects.

•        Stress. Use of IVF can be financially, physically and emotionally draining. Support from counselors, family and friends can help you and your partner through the ups and downs of infertility treatment.

How you prepare

When choosing an in vitro fertilization (IVF) clinic, keep in mind that a clinic's success rate depends on many factors, such as patients' ages and medical issues, as well as the clinic's treatment population and treatment approaches.

Before beginning a cycle of IVF using, you and your partner will likely need various screenings, including:

•        Ovarian reserve testing. To determine the quantity and quality of your eggs, your doctor might test the concentration of follicle-stimulating hormone (FSH), estradiol (estrogen) and antimullerian hormone in your blood during the first few days of your menstrual cycle. Test results, often used together with an ultrasound of your ovaries, can help predict how your ovaries will respond to fertility medication.

•        Semen analysis. If not done as part of your initial fertility evaluation, your doctor will conduct a semen analysis shortly before the start of an IVF treatment cycle.

•        Infectious disease screening. You and your partner will both be screened for infectious diseases, including HIV.

•        Practice (mock) embryo transfer. Your doctor might conduct a mock embryo transfer to determine the depth of your uterine cavity and the technique most likely to successfully place the embryos into your uterus.

•        Uterine cavity exam. Your doctor will examine your uterine cavity before you start IVF. This might involve a sonohysterography — in which fluid is injected through the cervix into your uterus — and an ultrasound to create images of your uterine cavity. Or it might include a hysteroscopy — in which a thin, flexible, lighted telescope (hysteroscope) is inserted through your vagina and cervix into your uterus.

Before beginning a cycle of IVF, consider important questions, including:

•        How many embryos will be implanted? The number of embryos implanted is typically based on the age and number of eggs retrieved. Since the rate of implantation is lower for older women, more embryos are usually implanted. However, most doctors follow specific guidelines to prevent a higher order multiple pregnancy — and in some countries, legislation limits the number of embryos that can be implanted at once. Make sure you and your doctor agree on the number of embryos that will be implanted before they're transferred.

•        What will you do with any extra embryos? Extra embryos can be frozen and stored for future use for several years. Not all embryos will survive the freezing and thawing process, although most will. Cryopreservation can make future cycles of IVF less expensive and less invasive. However, the live birth rate from frozen embryos is slightly lower than the live birth rate from fresh embryos.

•        How will you handle a multiple pregnancy? If more than one embryo is implanted in your uterus, IVF can result in a multiple pregnancy — which poses health risks for you and your babies. In some cases, fetal reduction can be used to help a woman deliver fewer babies with lower health risks.

What you can expect

In vitro fertilization (IVF) involves several steps — ovulation induction, egg retrieval, sperm retrieval, fertilization and embryo transfer. One cycle of IVF can take about two weeks, and more than one cycle may be required.

Ovulation induction

 You begin treatment with synthetic hormones to stimulate your ovaries to produce multiple eggs — rather than the single egg that normally develops each month. Multiple eggs are needed because some eggs won't fertilize or develop normally after fertilization.

You may need several different medications, such as:

•        Medications for ovarian stimulation. To stimulate your ovaries, you might receive an injectable medication containing a follicle-stimulating hormone (FSH), a luteinizing hormone (LH) or a combination of both. These medications stimulate more than one egg to develop at a time.

•        Medications for oocyte maturation. When the follicles are ready for egg retrieval — generally after eight to 14 days — you will take human chorionic gonadotropin (HCG) or other medications to help the eggs mature.

•        Medications to prevent premature ovulation. These medications prevent your body from releasing the developing eggs too soon.

•        Medications to prepare the lining of your uterus. On the day of egg retrieval or at the time of embryo transfer, your doctor might recommend that you begin taking progesterone supplements to make the lining of your uterus more receptive to implantation.


Your doctor will work with you to determine which medications to use and when to use them.

Typically, you'll need one to two weeks of ovarian stimulation before your eggs are ready for retrieval. To determine when the eggs are ready for collection, your doctor will likely perform:

•        Vaginal ultrasound, a procedure that uses sound waves to create an image of your ovaries to monitor the development of fluid-filled ovarian sacs where eggs mature (follicles)

•        Blood tests to measure your response to ovarian stimulation medications — estrogen levels typically increase as follicles develop and progesterone levels remain low until after ovulation

Sometimes IVF cycles need to be canceled before egg retrieval for one of these reasons:

•        Inadequate number of follicles developing

•        Premature ovulation

•        Too many follicles developing, creating a risk of ovarian hyperstimulation syndrome

•        Other medical issues

If your cycle is canceled, your doctor might recommend changing medications or their dose to promote a better response during future IVF cycles.

Egg retrieval

Egg retrieval can be done in your doctor's clinic 34 to 36 hours after the final injection and before ovulation.

•        During egg retrieval, you'll be sedated and given pain medication.

•        Transvaginal ultrasound aspiration is the usual retrieval method. An ultrasound probe is inserted into your vagina to identify follicles. Then a thin needle is inserted into an ultrasound guide to go through the vagina and into the follicles to retrieve the eggs.

•        The eggs are removed from the follicles through a needle connected to a suction device. Multiple eggs can be removed in about 20 minutes.

•        After egg retrieval, you may experience cramping and feelings of fullness or pressure.

•        Mature eggs are placed in a nutritive liquid (culture medium) and incubated. Eggs that appear healthy and mature will be injected with sperm to attempt to create embryos. However, not all eggs may be successfully fertilized.

Sperm retrieval

your partner will provide a semen sample at your doctor's clinic through masturbation the morning of egg retrieval. Other methods, such as testicular aspiration — the use of a needle or surgical procedure to extract sperm directly from the testicle — are sometimes required. Sperm are separated from the semen fluid in the lab.


Fertilization can be attempted using two common methods:

•        CIVF (Conventional In Vitro Fertilization). During insemination, healthy sperm and mature eggs are mixed and incubated overnight.

•        Intracytoplasmic sperm injection (ICSI). In ICSI, a single healthy sperm is injected directly into each mature egg. ICSI is often used when semen quality or number is a problem or if fertilization attempts during prior IVF cycles failed.

In certain situations, your doctor may recommend other procedures before embryo implantation.

•        Assisted hatching. About five to six days after fertilization, an embryo "hatches" from its surrounding membrane (zona pellucida), allowing it to implant into the lining of the uterus. If you're an older woman, or if you've have had multiple failed IVF attempts, your doctor might recommend assisted hatching — a technique in which a hole is made in the zona pellucida just before implantation to help the embryo hatch and implant.

•        Preimplantation genetic testing. Embryos are allowed to develop in the incubator until they reach a stage where one or two blastomeres removed and tested for specific genetic diseases or the correct number of chromosomes, Embryos that don't contain affected genes or chromosomes can be implanted in your uterus. While preimplantation genetic testing can reduce the likelihood that a parent will pass on a genetic problem, it can't eliminate the risk. Prenatal testing may still be recommended.

Embryo transfer

Embryo transfer is done at your doctor's clinic and usually takes place two to six days after egg retrieval.

•        The procedure is usually painless, although you might experience mild cramping.

•        The doctor or nurse will insert a long, thin, flexible tube called a catheter into your vagina, through your cervix and into your uterus.

•        A syringe containing one or more embryos suspended in a small amount of fluid is attached to the end of the catheter and then delivered through the tube into your uterus.

If successful, the embryo will implant in the lining of your uterus about six to 10 days after egg retrieval.

After the procedure

After the embryo transfer, you can resume your normal daily activities. However, your ovaries may still be enlarged. Consider avoiding vigorous activity, which could cause discomfort.

Typical side effects include:

•        Passing a small amount of clear or bloody fluid shortly after the procedure — due to the swabbing of the cervix before the embryo transfer

•        Breast tenderness due to high estrogen levels

•        Mild bloating

•        Mild cramping

•        Constipation

If you develop moderate or severe pain after the embryo transfer, contact your doctor. He or she will evaluate you for factors such as infection, twisting of an ovary (ovarian torsion) and severe ovarian hyperstimulation syndrome.


Your doctor will take a blood sample to detect whether you're pregnant about 10 days to two weeks after egg retrieval.

•        If you're pregnant, your obstetrician will continue with you for prenatal care.

•        If you're not pregnant, you'll stop taking progesterone and likely get your period within a week. If you don't get your period or you have unusual bleeding, contact your doctor. If you're interested in attempting another cycle of in vitro fertilization (IVF), your doctor might suggest steps you can take to improve your chances of getting pregnant through IVF.

The chances of giving birth to a healthy baby after using IVF depend on various factors, including:

•        Maternal age. The younger you are, the more likely you are to get pregnant and give birth to a healthy baby during IVF.

•        Embryo status. Transfer of embryos which are more developed is associated with higher pregnancy rates compared with less developed embryos (day two or three). However, not all embryos survive the development process. Talk with your doctor or other care provider about your specific situation.

•        Reproductive history. Women who've previously given birth are more likely to be able to get pregnant using IVF than are women who've never given birth. Success rates are lower for women who've previously used IVF multiple times but didn't get pregnant.

•        Cause of infertility. Having a normal supply of eggs increases your chances of being able to get pregnant using IVF. Women who have endometriosis are less likely to be able to get pregnant using IVF than are women who have unexplained infertility.

•        Lifestyle factors. Women who smoke typically have fewer eggs retrieved during IVF and may miscarry more often. Smoking can lower a woman's chance of success using IVF by 50 percent. Obesity can decrease your chances of getting pregnant and having a baby. Use of alcohol, recreational drugs, excessive caffeine and certain medications also can be harmful.


Talk with your doctor about any factors that apply to you and how they may affect your chances of a successful pregnancy.

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